Isolation and Characterization of Red Bean and Green Bean Protein using the Extraction Method and Isoelectric pH

Slamet Hadi Kusumah, Robi Andoyo, Tita Rialita

Abstract


Stunting children need food intake with higher protein and essential amino acids such as lysine and leucine than normal children. Red Beans (Phaseolus Vulgaris L.) and Green Beans (Phaseolus Radiatus L.) have protein content, respectively 22.07 ± 0.13% and 19.99 ± 0.07%, and are rich in essential amino acids lysine and leucine needed by a stunting child. The Development of food products with high protein concentrations (high protein food) such as isolates/protein concentrates is an important subject that must be done. This study aims to determine the technique of isolating red bean and green bean protein through extraction and deposition methods at isoelectric pH. The research method used is an experimental method with descriptive data analysis. Identification of isoelectric pH of red bean and green bean protein is done through electrophoretic mobility (cm2/Vs) testing on samples that have been conditioned at 3 different depositional pHs namely pH 3.00, 4.00, and 5.00. The results showed the isoelectric pH value of red bean and green bean protein, namely pH 4.56 and pH 4.81 respectively, where the pH had electrophoretic mobility values equal to zero. The process of isolating red bean and green bean protein produces protein powder with a protein content of 79.22 ± 0.06% and 80.69 ± 0.06%, respectively. Based on the weight yield, red bean and green bean protein concentrate powder had a yield of 14.88% and 16.75%, respectively.


Keywords


Protein Isolation; Extraction; Isoelectric pH; Red Beans; Green Beans.

References


Yusuf, A.A., Komarulzaman, A., Alisjahbana, A.S., Anna, Z., Ghina, A.A., & Megananda, A.S. (2018). Seri Menyongsong SDGs: Kesiapan Kabupaten/Kota di Provinsi Jawa Barat, Bandung: Unpad Press.

Semba, R. D., Shardell, M., Sakr Ashour, F. A., Moaddel, R., Trehan, I., Maleta, K. M., … Manary, M. J. (2016). Child Stunting is Associated with Low Circulating Essential Amino Acids. EBioMedicine, 6, 246–252. doi:10.1016/j.ebiom.2016.02.030.

Almatsier S., (2013) Prinsip Dasar Ilmu Gizi. Jakarta: PT. Gramedia Pustaka Utama, 2013.

Wang, M., Jiang, L., Li, Y., Liu, Q., Wang, S., & Sui, X. (2011). Optimization of Extraction Process of Protein Isolate from Mung Bean. Procedia Engineering, 15, 5250–5258. doi:10.1016/j.proeng.2011.08.973.

Gunarti, D. R., Rahmi, H., & Sadikin, M. (2013). Isolation and Purification of Thiamine Binding Protein from Mung Bean. HAYATI Journal of Biosciences, 20(1), 1–6. doi:10.4308/hjb.20.1.1.

Yi-Shen, Z., Shuai, S., & FitzGerald, R. (2018). Mung bean proteins and peptides: nutritional, functional and bioactive properties. Food & Nutrition Research, 62(0). doi:10.29219/fnr.v62.1290.

Kong, X., Li, X., Wang, H., Hua, Y., & Huang, Y. (2008). Effect of lipoxygenase activity in defatted soybean flour on the gelling properties of soybean protein isolate. Food Chemistry, 106(3), 1093–1099. doi:10.1016/j.foodchem.2007.07.050.

Dia, V. P., Wang, W., Oh, V. L., Lumen, B. O. d., & de Mejia, E. G. (2009). Isolation, purification and characterisation of lunasin from defatted soybean flour and in vitro evaluation of its anti-inflammatory activity. Food Chemistry, 114(1), 108–115. doi:10.1016/j.foodchem.2008.09.023.

Speroni, F., Milesi, V., & Añón, M. C. (2010). Interactions between isoflavones and soybean proteins: Applications in soybean-protein–isolate production. LWT - Food Science and Technology, 43(8), 1265–1270. doi:10.1016/j.lwt.2010.03.011.

Thenawidjaja, M., Wangsa, T. I., & Debbie, S. R. (2017). Protein Serial Biokimia Mudah dan Menggugah. Jakarta: Grasindo, 35.

Andarwulan, N., (2019) “Plant-Based Protein as Functional Food Ingredients.” in In Depth Seminar Internasional Foodreview Indonesia. Bogor, 14 Maret 2019.

Helvich, K. (1990). Official methods of analysis (No. 630.24 A88 1990). Association of official analytical chemists (AOAC). Available online: https://law.resource.org/pub/us/cfr/ibr/002/aoac.methods.1.1990.pdf (accessed 26 March 2020 ).

Association of official analytical chemists (AOAC), Tryptophan in Foods and Food and Feed Ingredient. Ion Exchange Chromatographic Method. AOAC International, 2005.

Rohman, A., & Gandjar, I. G. (2007). Metode Kromatografi Untuk Analisis Makanan. Yogyakarta: Pustaka Pelajar.

Andoyo, R., Guyomarc’h, F., Burel, A., & Famelart, M.-H. (2015). Spatial arrangement of casein micelles and whey protein aggregate in acid gels: Insight on mechanisms. Food Hydrocolloids, 51, 118–128. doi:10.1016/j.foodhyd.2015.04.031.

Lafarga, T., Álvarez, C., Bobo, G., & Aguiló-Aguayo, I. (2018). Characterization of functional properties of proteins from Ganxet beans (Phaseolus vulgaris L. var. Ganxet) isolated using an ultrasound-assisted methodology. LWT, 98, 106–112. doi:10.1016/j.lwt.2018.08.033.

Kanetro, B. (2017). Teknologi Pengolahan dan Pangan Fungsional Kacang-Kacangan, Yogyakarta: Palantaxia.

Du, M., Xie, J., Gong, B., Xu, X., Tang, W., Li, X., … Xie, M. (2018). Extraction, physicochemical characteristics and functional properties of Mung bean protein. Food Hydrocolloids, 76, 131–140. doi:10.1016/j.foodhyd.2017.01.003.

Winarno, F. G. (1991). Kimia Pangan Dan Gizi. PT Gramedia Pustaka Utama Press, Jakarta, Indonesia.

Kusnandar, F., Andarwulan, N., & Herawati, D. (2011). Analisis Pangan. Jakarta: Dian Rakyat.

Agostoni, C., Carratù, B., Boniglia, C., Lammardo, A. M., Riva, E., & Sanzini, E. (2000). Free Glutamine and Glutamic Acid Increase in Human Milk Through a Three-Month Lactation Period. Journal of Pediatric Gastroenterology and Nutrition, 31(5), 508–512. doi:10.1097/00005176-200011000-00011.

Hertz, L., Dringen, R., Schousboe, A., & Robinson, S. R. (1999). Astrocytes: glutamate producers for neurons. Journal of neuroscience research, 57(4), 417-428. doi: 10.1002/(sici)1097-4547(19990815)57:4<417::aid-jnr1>3.0.co;2-n.

Music, Denis, Damian M. Holzapfel, Felix Kaiser, and Erik Wehr. “Aspartic Acid Adsorption on Thermoelectric Surfaces.” Applied Surface Science 496 (December 2019): 143716. doi:10.1016/j.apsusc.2019.143716.

Akaerue, B. I., & Onwuka, G. I. (2010). Evaluation of the Yeild, Protein Content and Functional Properties of Mungbean [Vigna radiata (L.) Wilczek] Protein Isolates as Affected by Processing. Pakistan Journal of Nutrition, 9(8), 728–735. doi:10.3923/pjn.2010.728.735.

Hatti-Kaul, R., & Mattiasson, B. (Eds.). (2003). Isolation and purification of proteins. CRC Press.

Salgin, S., Salgin, U., & Bahadir, S. (2012). Zeta potentials and isoelectric points of biomolecules: the effects of ion types and ionic strengths. Int. J. Electrochem. Sci, 7(12), 12404-12414.

Mubarok, Z. R. (2019). Pengaruh Penambahan Asam Sitrat Pada Proses Perebusan Dan Perendaman Kedelai Untuk Mempercepat Proses Fermentasi Tempe. Jurnal Ilmiah Teknik Kimia, 3(1). doi:10.32493/jitk.v3i1.2596.

Coscueta, E. R., Campos, D. A., Osório, H., Nerli, B. B., & Pintado, M. (2019). Enzymatic soy protein hydrolysis: A tool for biofunctional food ingredient production. Food Chemistry: X, 1, 100006. doi:10.1016/j.fochx.2019.100006.


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DOI: 10.28991/SciMedJ-2020-0202-5

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